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Image Search Results
Journal: Applied and Environmental Microbiology
Article Title: Long-Term Survival of Campylobacter jejuni at Low Temperatures Is Dependent on Polynucleotide Phosphorylase Activity
doi: 10.1128/AEM.01366-09
Figure Lengend Snippet: (A) Genetic organization of the pnp CJJ81176_1270 cluster showing the putative transcriptional start site, localization of the insertion of aphA3 within the pnp gene, putative transcriptional terminator site, and position of the different primers used in this study. KnR, kanamycin resistance. (B) RT-PCR assays performed using C. jejuni 81-76 mRNA. RT experiments were performed with random hexamer primers, and PCR was done with the primer pairs MO005/MO006 (lane 1), MO007/MO006 (lane 2), MO005/MO008 (lane 3), MO007/MO008 (lane 4), and MO008/MO009 (lane 5). A 1-kb DNA ladder (BioLab) is shown on the left.
Article Snippet: This gene was PCR amplified from C. jejuni 81-76 chromosomal DNA obtained by a
Techniques: Reverse Transcription Polymerase Chain Reaction, Random Hexamer Labeling